Name of the lab
Apparatus
Vessels used for pre-culture
Total volume of the vessel (ml)
Type of vessel
Amount of medium in the vesssel (ml)
Surface size (x*y cm, diameter)
Depth of the medium (cm)
Covering type
Vessels used for test performance
Total volume of the vessel (ml)
Type of vessel
Amount of medium in the vessel (ml)
Surface size (x*y cm, diameter)
Depth of the medium (cm)
Covering type
Description of the spectrometer for chlorophyll if used:
Description of image processing unit if used
Description of luminometer to measure light intensity
In which unit illumination is measured?
Which kind of sensor is used?
Description of  pH-meter
Description of tools used to handle the duckweed fronds (tweezes, fork...)
Reagents
Dilution water (type, conductivity in µS/cm)
3,5-Dichlorophenol
Purity
Source
Charge number
other
Test organism Lemna minor
Use of Lemna minor ST (Y/N)
Use of other clone (Y/N)
if yes: Origin
Clone cultivated in lab conditions since (after taking from nature)
How and by whom was the clone characterised as Lemna minor?
Pre-culture
Please describe shortly how the stock culture is performed:
Please describe shortly how the preculture is performed:
Is the growth rate/multiplication controlled in the pre-culture?
If yes how is it done?
What is the growth rate/multiplication of the preculture?
What is the must abundant colony size in the preculture (1 frond, 2 fronds, 3 fronds, 4-5 fronds, more than 5 fronds)?
Estimated surface coverage in the preculture vessels
Are the precultures raised in the same climate chamber as the test or how are similar conditions achieved?
How often is the medium changed in the precultures?
How many days were between  taking the duckweed culture from agar/other stock culture and start of the test (<2 weeks, < 6 weeks, > 6 weeks)?
Test chamber
Please give a short  description of the test facility.?
Where and how in temperature measured?
How is temperature kept constant?
Medium temperature measured:
Deviation measured (+/-):
Did you ever perform a non-toxicant test?
If yes what was the coefficient of variation for growth rate measured  for all  positions used later?
If yes what was the maximum, minimum, medium value for light intensity  measured  for all  positions used later (µE m-2 s-1)?
If yes what was the maximum, minimum, medium value for temperature  measured  for all  positions used later (µE m-2 s-1)?
What kind of illumination is used?
Do you use a black bottom in the test chamber for all tests or only in case of turbid/coloured samples ?
Are there any methods used to minimise light from the side?
Media
Which test medium is used (Steinberg, APHA, SIS)?
If APHA is used for the test how many hours before the start of the test the change to Steinberg was made?
Which stock solutions were used/pooled for preparation of the Steinberg test medium if used?
Measured pH of the test medium before the start of the test?
Did you see any precipitation, milky clouds or change in colour of the sterile filter during storage of medium preparation?
Please describe if and how the medium was sterilised:
Stock solution of reference toxicant
Concentration of the reference toxicant used (mg/L)?
Use of solvents (yes/no)?
Statistics
Please give short descriopion of the methods used for calculating EC-values and confidence intervals.
Statistical program used
Calculation with commercial progam of own development within which software?
How are growth rates calculated
Which fit algorithm is used (if information available) for EC-values
How are confidence intervals calculated.
General deviations from the guideline
Comments on test performance
Comments on test results