Name of the lab
Test number
Start of the test
End of the test
Duration in hours:
Toxic substances used
Use of solvent (if yes with conc. In final medium in µL/L)
Type of medium used:
Number of control replicates
Number of treatment replicates
Number of additional replicates for establishing starting values for the 2nd parameter to be measured:
Do you use the same number of fronds and colonies for the inoculum in each vessel?
Number of fronds in the inoculum.
Number of colonies in the inoculum.
How long was the pre-culture under test-conditions before starting the test described on this sheet?
Medium temperature measured:
Deviation measured (+/-):
Deviation from Standard for this test:
Comment
				PLEASE LEAVE FIELDS EMPTY IF NO DATA ARE TO BE INSERTED.										Results	Please do not change any fields here.
				Please fill in all green and yellow fields and add comment o blue fields where appropriate
				There may be more concentrations and measurement times in this form than actually used.										Mean values
Raw data and results				Please leave superfluous field empty but do not erase them.
				Please comment at least every sample excluded for any reason but insert nevertheless values to the fields.
Observation parameter				All values filled in are dummies/default values.										Mean values	Frond number
Frond number	Time (h)		Treatment
	0	h          Conc.	Control	1	2	3	4	5	6	7	mg/L	Comment/add. observations				0	1	2	3	4	5	6	7	mg/L
		1	10	10	10	10	10	10	10	10					0	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		2	10	10	10	10	10	10	10	10					2	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		3	10	10	10	10	10	10	10	10					72	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		4	10												120	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		5	10												168	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		6	10
		#Replicates	6	3	3	3	3	3	3	3
		Mean	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		Std.Dev	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
		CV%	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
	2	h
		1	10	10	10	10	10	10	10	10
		2	10	10	10	10	10	10	10	10
		3	10	10	10	10	10	10	10	10
		4	10
		5	10
		6	10
		#Replicates	6	3	3	3	3	3	3	3
		Mean	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		Std.Dev	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
		CV%	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
	72	h
		1	10	10	10	10	10	10	10	10
		2	10	10	10	10	10	10	10	10
		3	10	10	10	10	10	10	10	10
		4	10
		5	10
		6	10
		#Replicates	6	3	3	3	3	3	3	3
		Mean	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		Std.Dev	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
		CV%	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
	120	h
		1	10	10	10	10	10	10	10	10
		2	10	10	10	10	10	10	10	10
		3	10	10	10	10	10	10	10	10
		4	10
		5	10
		6	10
		#Replicates	6	3	3	3	3	3	3	3
		Mean	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		Std.Dev	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
		CV%	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
	168	h
		1	10	10	10	10	10	10	10	10
		2	10	10	10	10	10	10	10	10
		3	10	10	10	10	10	10	10	10
		4	10
		5	10
		6	10
		#Replicates	6	3	3	3	3	3	3	3
		Mean	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		Std.Dev	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
		CV%	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
Results:	Results are recalculated centrally for specific ring test needs.
	As calculation of results is an important part of the whole ring test performance please add EC-values calculated by your own method generally used to the following tabel.
	Later comparison of own values with centrally calculated values will give additional unique information on own ring test performance.
	Please calculate growth rate values at least EC50 with confidence intervals and growth rate for the controls.
Lab name	0		Test No.	0
Observation parameter	Frond number
Parameter	EC5 (rate)	EC10 (rate)	EC20 (rate)	EC25 (rate)	EC50 (rate)
mg/L
lower 95%-cl
upper 95%-cl
lower 99%-cl
upper 99%-cl
				Participants are encouraged to measure more than two parameters (e. g. frond area and dry weight/chlorophyll)
				to provide a better survey on sensitivity even if this is NO part of the ring test necessities.
				For those labs measuring chlorophyll with a method providing not only chlorophyll a values please report
				raw data and EC values at least for chlorophyll a and total chlorophyll separately.
				In case of measuring destructive parameter with separate inoculum samples please insert these values in the										Results	Please do not change any fields here.
				control start values and copy mean value to all start values of the treatments.
														Mean values
Raw data and results
Observation parameter														Mean values	Second observation parameter
Second observation parameter	Time (h)		Treatment
	0	h          Conc.	Control	1	2	3	4	5	6	7	mg/L	Comment/add. observations				0	1	2	3	4	5	6	7	mg/L
		1	10	10	10	10	10	10	10	10					0	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		2	10	10	10	10	10	10	10	10					2	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		3	10	10	10	10	10	10	10	10					72	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		4	10												120	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		5	10												168	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		6	10
		#Replicates	6	3	3	3	3	3	3	3
		Mean	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		Std.Dev	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
		CV%	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
	2	h
		1	10	10	10	10	10	10	10	10
		2	10	10	10	10	10	10	10	10
		3	10	10	10	10	10	10	10	10
		4	10
		5	10
		6	10
		#Replicates	6	3	3	3	3	3	3	3
		Mean	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		Std.Dev	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
		CV%	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
	72	h
		1	10	10	10	10	10	10	10	10
		2	10	10	10	10	10	10	10	10
		3	10	10	10	10	10	10	10	10
		4	10
		5	10
		6	10
		#Replicates	6	3	3	3	3	3	3	3
		Mean	10,0	10,0	10,0	10,0	10,0	10,0	10,0	10,0
		Std.Dev	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
		CV%	0,0	0,0	0,0	0,0	0,0	0,0	0,0	0,0
	120	h
		1	10	10	10	10	10	10	10	10
		2	10	10	10	10	10	10	10	10
		3	10	10	10	10	10	10	10	10
		4	10
		5	10