High Content Phenomics

High-Content Screening
LemnaTec scanalyzer HTS technology

High-Content Screening

The term high-content screening originates in the pharmaceutical screening of new, active ingredients. Here, high-content screening is used to discriminate between tests with just one read-out parameter (often colour change of enzymatic or similar basic tests in multiwell plates of up to 1284 wells) and tests where each sample has multiple read-out parameters. Such high-content screening tests are mostly read out by imaging systems. The screening tests contain more complex model systems such as cell aggregations, or complete organisms such as the nematode Caenorhabtitis elegans.

High-content screening using LemnaTec scanalyzerHTS technology

Due to their high flexibility, the LemnaTec scanalyzerHTS units allow the performance of medical screening tests in multiwell plates of up to 96 wells. Especially for units with plant handling robots, plates of up to 396 wells can be read out with great flexibility and scalable magnification. This is possible even for graded systems, where all wells are assessed on low magnification first, to identify the interesting ones, which are then in turn reassessed at higher magnifications, e.g. for behavioral movement analysis. Since LemnaTec scanalyzer systems are such versatile, highly flexible, image analysis-based measurement systems, their technology can be similarly applied to pesticide high-throughput screening as well as high-content plant screening for agronomic and biological research.

High-content screening of cell aggregations and small organism testing extracts a maximum of pharmacological or physiological information from such screenings.