The LemnaTec Scanalyzer HTS (high throughput screening)

With the novel LemnaTec development Scanalyzer HTS image analysis can be performed as a detection method in High Throughput Screening. Together with the LemnaTec HTS Software, that is specially developed for the Scanalyzer HTS, the evaluation of biological and other tests based on the recognition of brightness, colour or shapes can be fully automatised. The yielded results are stored in a database according to GLP standards.
The Scanalyzer HTS analyses 500 microtiter plates in approx. eight hours. With its barcode/data matrix reader all wells are identified automatically.

Image 1: The Scanalyzer HTS for 12 microtiter plates. A unit with the messures of 1.2 x 2 meters can be charged with 60 plates.

The Scanalyzer HTS is designed to be an open system. As a quick and salutary reaction on new test requirements, changes in soft- or hardware are possible. All common formats of microtiter plates can be deployed. Further on new cut-outs depending on the desired optical resolution can be obtained simply by changing the c-mount lens on the 3 chip CCD camera (see table 1). The extensively configurable LemnaTec software requires just a state of the art PC, specially equipped by LemnaTec.

Table 1: Resolution of the raw-images in correlation to the cut-out (can be determined by choice of the lens) Wells per MTP Cut-out region Wells per cut-out Size of image [mm] Pixelsize[mm²]

Wells per MTP	frame	Wells per frame	Image size [mm]	Smallest detectable area [µm² ]
96	1/1	96	80 x 120	156
	1/4	24	20 x 30	39
	1/16	6	5 x 7,5	10
48	1/1	48	80 x 120	156
	1/4	12	20 x 30	39
	1/16	3	5 x 7,5	10
16	1/1	16	80 x 12	156
	1/4	4	20 x 30	39
	1/16	1	5 x 7,5	10

Image 2: Microtiter plates are analysed with transmitted or incident light

The construct made from inner-strutted aluminium components harbours robust precision components such as multiphase motors, 3-chip CCD cameras, lightning elements and barcode/datamatrix reader. The unit is CE certified. Not to be seen in pictures 1-3 a light tight cover as well as photo-electric guards at service and operation openings guaranty safe working conditions.The test organisms are benignly treated by minimal transfer of heat from the lighting elements and short times of scanning ( see table 2 ).

Table 2: Scanning timesamount MTPs Scans per MTP Wells per scan Scans overall Overall time of positioning[s] Scanning timezeit on plate [s] Barcode scan[s] Scanning time for 60 MTPs[min]

Amount of MTP	Scans per MTP	Wells per Scan	Scans over all	total positioning time[s]	Scanning time on plate[s]	Barcode Scan [s]	Scanning time 60 MTP [min]
60	1	96	60	1200	0	1200	40
60	4	24	240	1200	2700	1200	85
60	16	6	960	1200	13500	1200	265

Image 3: Side view on the Scanalyzer HTS

All original images are stored in a database linked together with the output of the LemnaTec picture analysis software, e.g. false-colour images, images of fluorescence, inhibition values and mobilities of organisms. This ensures high reproducibility, high availability of data and the option of data mining. ODBC and SQL interfaces take care of a performing communication in any intra-net.

Together with one or more LemnaTec software bundles the Scanalyzer HTS forms a complete unit for the evaluation of High-Throughput-Screening test. The following software is offered including the first configuration for your HTS test:

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The Scanalyzer HTS is available in 3 different sizes. (25 MTP, 50 MTP, 100 MTP) Other sizes can bee design on request.