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Tests were performed in 96 well plates but other multiwell plates would be suitable too. C. elegans was used as test organism in all sizes from adult ones to small juveniles as reproduction was observed during the time range of image acquisition. To provide acceptable living conditions plates were kept at room temperature (about 20 °C). For feeding a suspension of E. coli was provided. Test media was used corresponding to the German standard DIN draft of ecotoxicological nematode testing. Where dilution was necessary tap water was used. Image acquisition was performed at a range of different magnifications from 1 to 24 wells per image. Observation time was in the range between 10 and 100 seconds.

Using advanced image processing steps the moving organisms can be extracted from each image of an image series acquired. This series of recognised moving nematodes can be used as basis for detailed movement or behavioural analysis if single nematodes should be analyzed in detail.One example for this is shown on the following images. While particles and debris did not

Above: original Right above: only moving objects anayzed. Right: movement quality