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High-Throughput Plant Phenotyping – Data Acquisition, Transformation, and Analysis

Bioinformatics Tools and Applications for plant phenotyping

Data Handling and Data mining is the most challenging task in modern Plant breeding using Plant Phenomic technologies. In Cooperation with Keygene (http://www.keygene.com) scientist José Lima-Guerra LemnaTec CEO Matthias Eberius have published a detailed information on how LemnaTec´s Plant Phenotyping Platform can help researchers to understand this challenge.

High throughput phenotyping for measuring drought tolerance

InterDrought III – Shanghai ‐ October 2009

High throughput phenotyping for measuring drought tolerance

Genomics has accelerated gene discovery due to high throughput techniques and robotics. Physiological characterization of plants is still time consuming and labor intensive.

 

  •  Phenotyping is essential for

– functional analysis of specific genes
– forward and reverse genetic analyses
– production of new plants with beneficial characteristics

High throughput phenotyping and plant modelling

two legs for combined physiological and genetic approaches ?

Phenotyping is the bottleneck in genomics.

  • Sequences of Arabidopsis, Rice, Sorghum, poplar, tomato, maize etc
  • Genotyping capacities for 1000s of genotypes
  • Large collections of RILs, mutants, accessions

Is this the proper way to address the problem ? (defined by a need, not by a biological question) Are "genotyping" and "phenotyping" parallel activities ?

The 'problem' is that we can now measure traits in1000s of plants in a robotised way

We need to refine biological questions avoiding reinventing the wheel

Modelling can help...

Quantifying the three main components of salinity tolerance in cereals

The Australian Centre for Plant Functional Genomics and the University of Adelaide, PMB1, Glen Osmond, SA 5064, Australia

Salinity stress is a major factor inhibiting cereal yield throughout the world. Tolerance to salinity stress can be considered to contain three main components: Na+ exclusion, tolerance to Na+ in the tissues and osmotic tolerance. To date, most experimental work on salinity tolerance in cereals has focused on Na+ exclusion due in part to its ease of measurement. It has become apparent, however, that Na+ exclusion is not the sole mechanism for salinity tolerance in cereals, and research needs to expand to study osmotic tolerance and tissue tolerance.

Agrobios,un ROBOT per studiare lo sviluppo delle piante tra GENOTIPO e caratteristiche ambientali

Le tecniche di analisi di immagine possono fornire importanti informazioni se applicate direttamente sulle piante. Infatti, l’analisi multispettrale (infrarosso, visibile e ultravioletto) della luce riflessa da chiome, fusto e foglie, è in grado di ricavare informazioni circa lo stato nutrizionale, idrico e patologico di una pianta, nonché sulla sua capacità di intercettazione della luce.

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