Non-destructive repeated phenotyping in vegetable crops using digital image analysis

Ralph van Berloo, José Guerra

• The importance of phenotyping
• Subjective observations
• Keygene & phenotyping
• Digital image analysis and the LemnaTec system
• Examples of digital imaging of vegetable crops & challenges encountered
• Conclusions and future directions
• Acknowledgements

The importance of phenotyping

• For plant breeders (and growers and consumers) the phenotype is the final goal..
• New improved phenotypes can be created through genetic improvement (QTLs)
• Detection of QTLs requires:
  • high quality molecular markers
  • high quality phenotypes !
• But ..Phenotyping is not always straightforward
  • Genetic and environmental factors are intertwined
  • Subjective/ biased observations
  • Phenotype may depend on complex interactions of plant and (a) biotic agent (resistance, tolerance)

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