Overcoming the phenotyping gap - experiences with the LemnaTec-Scanalyzer 3D platform investigating drought tolerance in barley
The LemnaTec-Scanalyzer 3D system in the IPK Gatersleben was established in 2008 and was one of the first LemnaTec-platforms in the public domain. Since then it is used for phenotyping drought tolerance of different barley cultivars. Plants are phenotyped fully automated with three different camera systems using I) visible light, II) fluorescence, and III) near infrared. In the beginning, several replications of a vegetative drought stress experiment were conducted using parents of a DH-population Morex and Barke, for testing the reproducibility of phenotypic values with the Scanalyzer system and gaining experiences. Since autumn 2010, the IPK is involved in the CROP.SENSe.net project and drought stress is applied to a barley core set of eight old and eight modern german spring barley cultivars. The set shows a good phenotypic diversity in growth under control and drought stress conditions. In future, a larger set of a worldwide spring barley collection will be phenotyped for a genome-wide association study.
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