Non-destructive Plant Phenotyping
Non-destructive plant phenotyping is a key component of any phenotyping with comparatively low plant numbers (compared to field conditions) under highly controlled environmental conditions in greenhouses or growth chambers. These methods have the huge advantage of not destroying or harming test objects, and they therefore allow monitoring plants over their entire growth period. As a result, the plants act as their own control, eliminating all variability normally caused by harvesting of subsets and thus enhancing statistical data quality. Besides, plants are often far too valuable to be destroyed (up to 700 $ per piece), or even, depending on their origin in breeding programmes, simply unique and thus irreplaceable.
Non-destructive plant phenotyping, combining the best of image-based analysis with additional techniques, is the key concept of the LemnaTec scanalyzer3D and scanalyzerHTS systems.
Non-destructive plant phenotyping using LemnaTec scanalyzer technologies
Non-destructive Plant Phenotyping - LemnaTec scanalyzer systems provide a wide range of non-destructive methods to characterise plant phenotypes. Imaging methods using specific cameras for visible light (scanalyzerVIS), near infrared light (scanalyzerNIR) or infrared light (scanalyzerIR) for both shoots and – if applicable – roots provide fast data acquisition at high spatial resolutions. Hyperspectral imaging can increase the spectral resolution even further. Specific imaging modes such as fluorescence imaging add specific information to chlorophyll fluorescence, chemical markers or genetically initialised fluorescent markers such as GRP, YFP or RFP.
Water usage and evaporation is monitored by automated LemnaTec weighing and watering stations. Additional sensors, for example for soil humidity and leaf thickness, can complement individual plant measurements at very high time resolutions. All these data can be correlated to environmental parameters that are measured in the greenhouse and used for example to normalise growth data based on standard developmental models.
Non-destructive Plant Phenotyping, leaf identification
Non-destructive Plant Phenotyping, shape analysis