LemnaTec is constantly expanding human vision through imaging automatisation, mainly by employing various fully controlled cameras in compliance with industrial standard. Wherever suitable these are equipped with high-quality macro or zoom lens systems. Camera resolutions are optimised for different purposes and range from 1.3 to 10 megapixels per image for visible imaging on 1/2’’ specially selected chips. These are the true source of perfect image quality! As a result of taking one image per well, a 96-well plate can, for example, be imaged with minimal distortion as a 100 megapixel image!
NIR- and IR-cameras have a much lower resolution in the range of 0.1 to 0.3 megapixels due to the significantly different and more complex chip technology. Their advantage lies in the fact that they allow a high degree of automatisation and reproducibility of industrial standard technology.
Depending on the imaging system, visible light camera modules can be offered with all types of LemnaTec systems, both for top and backlight illumination. For 96-well plates specific dark field illuminations for each single well and also for larger areas of up to 10 cm in diameter are available.
Near infrared cameras are employed mainly for the visualisation of water in the 1450–1550 mm band, but they can also be adapted to other ranges between 900 and 1750 nm. Specific illuminations are available both for HTS systems (10*10 cm) and phenotyping systems (1.5*2.5 m).
High-throughput 3-D imaging systems provide a unique chance to quantify differences in leaf temperature, either within one plant or even between various plants, under highly controlled and automated conditions. This opens up new perspectives for the testing of extremely variable plant parameters, even allowing the identification of plants with unusual reaction patterns towards climate conditions or drought stress.
Visible light cameras with high sensitivity are combined with a unique filter combination for light and camera. Based on this technology any fluorescence excitable by blue light with sufficient emission (< 500 nm) can be visualised both in 2-D and 3-D systems under backlight or reflective conditions. This visualisation method can be applied to a wide range of test objects, from very small organisms to fully grown crop plants, by employing different types of GFP, YFP, RFP, chlorophyll, phenolics and other plant ingredients.
Assessment of soil water content in transparent root columns supplies dynamic information on the root’s water extraction efficiency concerning different soil layers. This provides valuable complementary information in addition to the basic root architecture, as root density is often badly correlated to the soil layers that plants prefer to extract water from.
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in Cooperation with Headwall we have integrated this unique technology into our Scanalyzer Platform for High throughput Plant Phenomics and High throughput Screening.
This technology will enable researchers to get a complete new view on their organisms. To improve traits like:
- Abiotic / biotic stress
- Salt and drought tolerance
- Nutrient use efficiency
- Water use efficiency
- Disease screening
- Root development
- Plant growth characteristics
- Response to chemical treatment
At the heart of the Hyperspec® Inspector system is a fully reflective, F/2 aberration-corrected imaging-spectrograph covering the VNIR spectral region of 400-1000 nm. Headwall Photonics has developed a family of such HyperspecTM spectrographs, each optimized for different spectral regions; UV/VIS (250-600 nm), VNIR (400-1000nm), Extended-VNIR (550-1700 nm), NIR (900-1700 nm) and SWIR (1000-2500 nm). For reference, all Headwall Hyperspec® sensors utilize the company’s patented aberration-corrected all-reflective Offner design, containing a high-efficiency cCubeonvex diffraction grating and suitable collimating and imaging mirrors. The fully reflective nature of the off-axis Offner design, and the absence of transmissive optics within the spectrograph module, ensures superior imaging performance. This is defined by the virtual absence of smile and keystone aberrations, as well as chromatic errors caused by transmissive optics, and ensures that the spatial and spectral fidelity of the hyperspectal image is fully and accurately characterized. The use of original diffraction grating ensure excellent low stray-light performance.
The latest technology available in a scientific grade CMOS (sCMOS) FPA detector is integrated in the Hyperspec® Inspector E-Series sensor. This fully embedded and ruggedized sCMOS camera has been developed for high speed, high dynamic range applications. The dimensions of the FPA are matched to the spatial and spectral dimension required for high speed industrial operation.