Particularly in the medical sector the term high-content screening is used to distinguish between an assessment of a single parameter tested in high-throughput or a comprehensive analysis of a broad set of parameters for each single sample. While such analysis is relatively new in the medical area, it is already well established for image-based assessments LemnaTec is performing in other research areas. At any rate LemnaTec extracts between 5 and 50 parameters from each image, always offering the customer the possibility to calculate even more, based on single images, several images made at one point in time (e.g. with image-based plant volumes) or based on several points in time (growth rates). In all cases the aim of comprehensive quantitative measurement of whole assays or single organism parameters is to deliver a maximum amount of relevant information to the customer.
The results of such multiple parameter assessments for the propagation of research have even entered standardised ecotoxicology, where leaf number AND frond area need to be measured for the subsequent calculation of inhibition by toxicants. For the phenotyping of plants with Scanalyzer 3D systems, the use of size, shape and colour information forms the basis for any further statistical analysis.
Right from the beginning LemnaTec has adapted the high-content analysis to the specific degree of throughput that the customer really needs, which may range from 20 beakers or plants per day to up to ten thousands of plants in trays on conveyor belt systems or in multiwell plates in Scanalyzer HTS systems.