High-throughput screening

High-throughput screeningIn all cases where large numbers of samples need to be assessed within short time periods, LemnaTec HTS systems can be applied to the needs of most agrochemical companies and specialised screening facilities. Most tests use multiwell plates between 6 and 96 wells.

The LemnaCount software package provides the framework – a reliable quantification of high-throughput screening tests for non-mobile organisms such as fungi on leave discs, artificial media germinating seeds, small plants, immobile aphids, diet- or leaf-feeding assays, and population tests with any kind of small organisms (mostly insects). Depending on the type of test a broad set of parameters, e. g. number, size, colour or covered area/size is measured. These parameters provide a comprehensive quantitative response pattern against toxicants, more than meeting the requirements of conventional yes/no- or classifying schemes.

In many cases screening tests require quantitative assessment of organism movement, which is quite difficult to perform by eye and leads to inaccurate results, particularly if the assessment carried out by more than one person. Application of multiwell tests using daphnia, mosquito larvae, nematodes, fish eggs/larvae or any other kind of small aquatic or terrestrial organisms proves an easy task when it can be automated. The LemnaTrack software package offers several options, either to differentiate between moving and non-moving organisms and/or to quantify movement, even in very confined vessels. Furthermore, size or colour parameters might be measured.

High-throughput screeningIn addition to image analysis, LemnaTec provides positioning inlays for the specific test vessels. Consultancy for result interpretation can also be supplied, if desired. Data is stored consistently, in accordance with quality insurance protocols, in the LemnaBase database. Raw data can be further transformed mathematically into e. g. growth rates and inhibition values by using the LemnaMiner. The results can then be reorganised into exportable files for further processing with specific statistical programmes. Alternatively, data can be directly transferred to other databases by customised data exchange protocols.

The use of the LemnaTec Scanalyzer HTS allows testing in a wide range of vessels, from petri dishes and multiwell plates of up to 96 wells to multi-compartment trays of customised size filled with soil. In all cases backlight, top light, dark field illumination or customised light sources may be implemented. If necessary, movement can be induced in mobilised organisms like aphids by using UV-light or heat sources.

The immense flexibility of the LemnaTec hard- and software systems represents an investment targeted at the future, even under changing requirements.

Hardware

  • LemnaTec scanalyzer3D systems open new prospects for the quantitative, non-destructive analysis of different crops or model plants under high-throughput phenomics conditions.

  • Teaser: The scanalyzer HTS is the best option for the analysis of small plants in multiwell plates or trays in specific growth chambers.

Software

  • Central Database Interface

  • LemnaGrid image analysis grids can be supplied online, as well as shared and exchanged, by using the LemnaGrid exchange interface.

  • Monitoring the fully integrated automatisation of all LemnaTec scanalyzer systems.

  • This comprehensive and intuitive interface manages all LemnaTec products.
     

  • The LemnaMiner extracts data from -LemnaTec databases and employs user-friendly tools to organise and visualise them.

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