APPLICATION CASE

Arabidopsis Phenotyping

Arabidopsis phenotyping aims at measuring phenotypic properties of Arabidopsis shoots, i.e., the rosettes. Typically plants are grown in trays, and individual plants are placed in regular patterns, for instance in rows and columns. The analysis delivers data per plant. Once time series of such trays are taken, growth and development can be monitored. Data comprise rosette area, rosette morphology, e.g., compactness, or surface colors. Arabidopsis phenotyping is carried out in various application ranges in plant science, for instance in studies on gene functions, in plant-environment assessments, or to rate treatment effects.

Exemplary fields of application

  • Plant development
  • Genotype-phenotype analysis
  • Responses to environment
  • Stress

Application ranges

  • Plant research
  • Plant genetics
  • Environmental research
  • Stress and disease phenotyping
  • Product development

Samples and imaging

  • Plants in trays arranged in regular patterns

  • Top view image
  • RGB imaging
  • White LED light

Analytical Software

  • Analysis programming in LemnaGrid
  • Analyses run with LemnaExperiment

Results and output parameters

  • Plant area from multiple views combined to digital biomass

  • Morphological factors (convex hull, enclosing circle, compactness etc.)

  • Rosette diameter and circumference

  • Analysis can be augmented, e.g., for color analysis, detection and quantification of symptoms caused by stress and diseases, or user-defined analysis targets.

  • Application dedicated to Arabidopsis, but can work with other rosette forming species or young seedlings, too.

Works with

PhenoAIxpert
  • PhenoAIxpert
  • HyperAIxpert

  • PhenoAIxpert Pro (not prime scope of the instrument; adaptations might be required)

  • PhenoAIxpert Flex (not prime scope of the instrument; adaptations might be required)

  • PhenoAIxpert HT / HTC (not prime scope of the instrument; adaptations might be required)