APPLICATION CASE

Chlorophyll Fluorescence Phenotyping

Chlorophyll Fluorescence serves as measure for the status and activity of the photosystem II. Analyzing images of chlorophyll fluorescence delivers photosynthesis-related data at whole-plant level. Measurements provide basic and maximum fluorescence of dark (F0, Fm) or light (F, Fm’) adapted plants. These factors serve to calculate parameters such as Fv/Fm, NPQ, ETR and more. In phenotyping, such analyses are used to rate the physiological status of plants and to figure out the effects of stress, diseases, or environmental factors.

Exemplary fields of application

  • Plant development
  • Photosynthetic parameters
  • Responses to environment and treatments
  • Stress
  • Diseases

Application ranges

  • Plant research
  • Plant breeding
  • Environmental research
  • Stress and disease phenotyping
  • Product development

Samples and imaging

  • Top view image

  • Plants in trays or on the ground, preferredly arranged in regular patterns

  • Plants in pots or containers

  • PAM protocol imaging

  • Kautsky (OJIP) protocol imaging

Analytical Software

  • Analysis programming in LemnaGrid
  • Analyses run with LemnaExperiment

Results and output parameters

  • Color coded representation of fluorescence data

  • Fv/Fm
  • Fv’/Fm’
  • NPQ
  • ETR
  • broad range of parameters to calculate

  • Indices can be calculated for virtually any plant species, including major crops and wild species.

Works with

  • HyperAIxpert
  • PhenoAIxpert CF

  • PhenoAIxpert HT

  • CanopyAIxpert

  • FieldAIxpert