APPLICATION CASE

Root Phenotyping

using images taken from flat rhizotrons

Root phenotyping – usually combined with shoot phenotyping – of plants grown in rhizotrons combines an access to the root system with a soil-based cultivation. It enables monitoring root growth, root system properties, and detemining the root density in soil layers. Analytical factors comprise root length, root system width and depth, root branching, root growth direction, root curvature, or root density per soil layers. Root phenotyping serves in genotype-phenotype studies, or in the assessment of plant responses to environmental factors. Root phenotypes are important in plant breeding, particularly for breeding plants adapted to harsh climatic conditions.

Exemplary fields of application

  • Root development
  • Root-shoot relations
  • Genotype-phenotype analysis
  • Responses to environment
  • Stress

Application ranges

  • Plant research
  • Soil research
  • Stress and disease phenotyping
  • Breeding
  • Product development
  • Fertilizers, Biostimulants, Agrochemicals

Samples and imaging

  • Plant grown in soil-filled rhizotron that is stored in a 45° angle

  • Root system visible at the transparent plate of the rhizotron

  • Image of the rhizotron plate for root phenotyping

  • Root image usually taken together with top- and side-view images of the shoot (shoot analysis shown in separate application cases)

  • RGB imaging
  • LemnaTec Specialized Rhizotron LED Array

Analytical Software

  • Analysis programming in LemnaGrid
  • Analyses run with LemnaExperiment

Results and output parameters

  • Root length
  • Root system width and depth
  • Root system area
  • Root system geometry
  • Rooting depth
  • Root density
  • Root branching and angles
  • Root curvature
  • Root growth direction
  • Applicable to many species

Works with

  • PhenoAIxpert Pro Root

  • RootAIxpert HT