Application Cases
Chlorophyll Fluorescence Phenotyping
Category: Leaf- and Shoot Phenotyping, Physiological Phenotyping, Stress and Disease Phenotyping
Chlorophyll Fluorescence serves as measure for the status and activity of the photosystem II. Analyzing images of chlorophyll fluorescence delivers photosynthesis-related data at whole-plant level. Measurements provide basic and maximum fluorescence of dark (F0, Fm) or light (F, Fm') adapted plants. These factors serve to calculate parameters such as Fv/Fm, NPQ, ETR and more. In phenotyping, such analyses are used to rate the physiological status of plants and to figure out the effects of stress, diseases, or environmental factors.
Vegetation Indices
Category: Leaf- and Shoot Phenotyping, Physiological Phenotyping, Stress and Disease Phenotyping
Vegetation indices can be calculated from multispectral or hyperspectral measurements. A vegetation index is calculated from the spectral reflectance of a plant sample at two or more wavelengths. Such indices serve to characterize developmental or physiological properties of the plants. For instance, greenness indices relate to chlorophyll abundance, pigment-related indices refer to e.g., anthocyanin or carotenoids, or stress-related indices are responsive to biotic and abiotic stress factors. Dozens of indices are reported in the literature, and it is possible to establish customized indices, too.
Morphology and Color Phenotyping
Category: Leaf- and Shoot Phenotyping, Stress and Disease Phenotyping
Color phenotyping delivers information about the plant surface and the colors. In particular, greenness is informative on chlorophyll abundance. Colors can also indicate stress, such as yellowing or browning that relates to wilting. Moreover, many pathogen infections are linked to characteristical colors. In combination with data on morphology of the plants, color data provide an insight in the developmental status. They can be used in genotype-phenotype studies as well as in the assessment of environmental influences or treatments.
Multiview Structural Phenotyping
Category: Leaf- and Shoot Phenotyping, Stress and Disease Phenotyping
Multiview phenotyping serves to determine plant dimensions and structure. Viewing the plants from multiple angles delivers comprehensive information on the structure and morphology of the plant. Data can be used to determine digital biomass. In time series, the method can serve to determine growth and development. The method not only serves to address the impact of environmental factors on plant development, but it is also applied to analyze the genetic control of growth and development.