The PhenoAIxpert ES is an advanced imaging solution for detection of bioluminescence effects. Bioluminescence is a widely used reporter for gene activity, molecular interactions, or plant-microbe interactions. Imaging of bioluminescence enables time- and space-resolved monitoring of presence, intensity, and localization of luminescence signals in the sample material. The bioluminescence imaging sensor is combined with high-resolution RGB imaging for standard phenotypic assessments of the analyzed plant material. Images from both sensor systems can be superimposed, thus allowing the analysis of bioluminescence in combination with classical phenotyping. The PhenoAIxpert ES has a high-sensitivity high-resolution imaging sensor with a deep-cooled vacuum CCD sensor. Being one of the most sensitive CCD cameras in the world, the system provides optimized photon collection properties and allows for precise recording of weakest light emissions. The camera is dedicated for long exposure times. The second camera mounted in the PhenoAIxpert ES is a >12 MPixel RGB industrial grade camera combined with optics and LED illumination optimized for top-view phenotyping of the plant material.