The versatile phenotyping system for laboratories

The PhenoAIxpert product family combines powerful analytical software with a range of hardware options. Thereby it enables reproducible and standardizable results in plant research, breeding, product development, quality assessment, and beyond.

Features

  • Image acquisition, storage, and advanced image analysis including machine learning options

  • Metadata, annotation, and analysis directly linked to image

  • Intuitive graphical user interface

  • Advanced LED illumination options

  • Optional optical filters

  • The laboratory phenotyping system is available with industry-grade cameras in horizontal, vertical, and rhizobox-view positions, combined with different lighting and imaging technologies

  • Phenotypic data include count, size, morphology, and color, as well as application-specific features

  • Easy export of images and data

Download the “PhenoAIxpert Pro Root” product brochure as a PDF document.

Application examples

Root Phenotyping

Using the PhenoAIxpert Root, traits of soil-grown roots in rhizotrons can be measured. Root properties like root lenght, root density, or root system lenght and width can be assessed.

Roots in Rhizotron at Growscreen RhizoRoots in Rhizotron at Growscreen Rhizo anaylsed with LemnaTec Software

Software Package

The PhenoAIxpert Pro Root is delivered with a comprehensive software package. The software enables the users to provide sample information and control cameras and lights. After imaging all data are stored and accessible for image and data processing.

LemnaControl is the interface with which users operate the system. With LemnaControl, users carry out all settings that are required for image recording with the system. In particular, this comprises settings for the camera(s) and lights included in the system. All settings can be combined and scheduled to configure recordings. The available functions are tailored to the corresponding hardware. Each hardware system delivered by LemnaTec comes with its own control software adapted for the functions of that system.

The LemnaGrid software module uses an intuitive graphical programming environment, which allows for easy integration of different image analysis algorithms. LemnaGrid phenotyping software offers high flexibility for user programming. The image processing workflow extracts desired properties/features from the original image and stores results in a dedicated storage system. LemnaGrid can include functions of state-of-the-art machine learning tools, next to standard image analysis to detect and extract features of interest. Guided by the LemnaGrid graphical user interface, users can build their own analytical workflows with pre-defined devices.

Analysis devices include:

  • filters

  • threshold functions

  • image converters

  • object detectors

  • feature detectors

  • color analysis tools

  • region of interest function

  • combinatory functions

  • and many more

All devices – graphically represented by boxes – can be combined on a interface by aligning the boxes to workflows. Along such workflows, each function can provide insights in the progress of the analysis by feeding out an intermediate image. Image processing workflows can include branches and parallel paths that combine to a final result. LemnaExperiment serves to manage experiments and measurements, and to schedule and run analyses. Moreover, it enables viewing, visualizing, and exporting results. It provides capabilities to access and browse through recorded images and to display metadata for samples and measurements. As core function, LemnaExperiment selects recordings for analysis and assigns an image analysis pipeline –written in LemnaGrid or in Lemna3D – to a given set of recordings. After assignment, the analyses are run in the background by the Analysis Executor. Parameters of interest can be selected in LemnaExperiment so that they are displayed in the analysis output.

Imaging and Lighting Options

Standard RGB imaging for shoot and root

  • Minimum 12 MPix RGB industrial camera with prime lens for distortion-free imaging

  • Optimized white LED illumination

Shoot imaging: Multispectral and fluorescence imaging options

Multispectral and fluorescence imaging is available for PhenoAIxpert shoot imaging using multi-channel LED
panels combined with a dedicated camera and filter system.

  • 16 MPix Greyscale camera with prime lens for distortion-free imaging

  • LEDs with a range of different wavelengths – camera
    images 16 MPix per channel
  • Filter wheel for up to 6 different filters for filtering
    reflected or emitted light
  • Imaging setup customized for rhizotrons

Wavelength ranges of LEDs in the systemIn a typical setup, the LED array consists of UV, blue, green, yellow, red, deep red, far red and near-infrared wavelength LEDs. Thereby, an advanced analysis of the red edge is enabled as different images in the red range are provided and can be used for ratio calculation. The filter wheel enables filtering for reflectance or fluorescence, e.g. in the chlorophyll fluorescence range.

Multi-channel LED illumination combined with filters enables various imaging settings

  • Channel-wise imaging

  • Combination of channels

  • Chlorophyll autofluorescence

  • Fluorescent biomarkers (e.g. GFP)

  • NDVI and other indices

  • Fluorescence imaging

Hardware

  • Rhizotron imaging option in PhenoAIxpert Pro cabinet

  • Top, side, and root view imaging and phenotyping

  • Simultaneous root- and shoot-imaging of plants

  • Phenotyping of soil-grown roots in high-quality rhizotrons

  • Reflection-minimized LED illumination for rhizotron imaging

  • Options: multispectral and fluorescence imaging for shoots

Optional Accessories (all versions)

  • Barcode reader

Future features

  • Database connectivity

  • Cloud services

We bought a LemnaTec LabScanalyzer (the predecessor of PhenoAIxpert) in July 2018. The instrument was delivered to us and installed remotely by a Lemnatec technician that instructed us about the use of the instrument. We were happy about the service: very good communication and excellent instrument set-up. The Lemnatec LabScanalyzer works nicely and is even more user-friendly than we expected. Almost no training of new users is needed. We hope to expand the uses of the instrument to obtain additional phenotyping options in the future, by interacting with LemnaTec.

Prof. Pierdomenico Perata, School of Advanced Studies, Pisa, Italy, Institute of life science

LemnaTec Product Matrix

LemnaTec offers imaging and image processing technologies for several applications that suit for low to high throughput in laboratory, greenhouse or outdoor environments. Technologies are adapted to various sample types and can be combined with automation. All imaging systems are combined with dedicated image processing software, including user-programmable functions and machine learning.

Ask for a quotation or additional information