The versatile phenotyping system for laboratories – advanced phenotyping of growing plants.

  • For many sample types in plant research, breeding and beyond

  • Growth assays, stress responses, quality assessments

  • Genotype-phenotype relations, genetic screenings

  • Disease- and toxicology assessments

  • 12 Megapixel industrial RGB cameras with high-quality optics in horizontal and vertical view

  • Illumination from top and side

  • Alternative imaging setup: multi-channel LEDs with greyscale camera for multispectral imaging

  • Advanced image processing algorithms, optional machine learning

  • Application-oriented customizable analyses

Application examples

Plant TrayPhenotyping Arabidopsis

Arabidopsis growth assay

Trays with growing Arabidopsis plants were imaged with PhenoAIxpert and images were processed for plant growth, morphology and color. Individual plants were identified and phenotypic properties of their visible plant area were assessed. Such tests serve for e.g. candidate screening, genetic studies, treatment effects, environmental responses

Bean Phenotyping

Bean plants were exposed to stress and phenotyped for stress responses. Images were recorded and processed with PhenoAIxpert Pro. This system enables vertical and horizontal multi-view imaging. Phenotypic traits comprise growth, morphology, and color analysis, e.g. as stress indicator for chlorosis and necrosis.

Phenotyping Bean Plant

Multispectral imaging example with intact leaf (left) dead dry leaf (right) and stress-damaged leaf (bottom)

Red, green, and blue channel imaging – color-coded visualization of reflectance, channels allow calculating RGB images.

Multispectral imaging example with intact leaf (left) dead dry leaf (right) and stress-damaged leaf (bottom)

Combining red and infrared image (color-coded visu-alization of reflectance) to NDVI image (greyscale in-tensity of NDVI values: light-grey high NDVI, dark-grey low NDVI)

Root Phenotyping

Using the PhenoAIxpert Root, traits of soil-grown roots in rhizotrons can be measured. Root properties like root lenght, root density, or root system lenght and width can be assessed.

Roots in Rhizotron at Growscreen RhizoRoots in Rhizotron at Growscreen Rhizo anaylsed with LemnaTec Software

Phenotyping of seedlings on petri dish

Assessing shoot and root traits of agar-grown seedlings with PhenoAIxpert: shoots and roots are recognized separately and measured for their individual size. In the current example, primary root length, secondary root length, and shoot area were measured for each seedling.

Seedlings on Agar ImagingSeedlings on Agar Phenotyping

Features

  • Reliable technology, advanced image processing

  • Phenotypic data include count, size, morphology, and color

  • Image acquisition, storage, and analysis
  • Metadata, annotation, and analyisis directly linked to image

  • Intuitive graphical user interface
  • Structured image file administration system

  • Easy export of images and data

Technical Data

  • PhenoAIxpert Pro – top and side view imaging and phenotyping

  • Horizontal and vertical views of plants, and multi-view imaging using a turntable for pots

  • Sample height up to 400 mm on turntable; alternative version with recessed sample holder for 600 mm sample height (no turntable)

  • Optional: multispectral LEDs and cameras with filter changer

  • Cabinet size 800 mm x  1800 mm footprint, 1780 mm height, 475 kg.

PhenoAIxpert Pro

Optional Accessories for PhenoAIxpert Pro

  • Sample holders for pots—easy positioning of the samples

  • Barcode reader

Download the “PhenoAIxpert Pro” product brochure as a PDF document.

Imaging and Lighting Options

Standard RGB imaging

  • Minimum 12 MPix RGB industrial camera with prime lens for distortion-free imaging
  • Optimized white LED illumination

Multispectral and fluorescence imaging options for the PhenoAIxpert family

Multispectral and fluorescence imaging is available for PhenoAIxpert shoot imaging using multi-channel LED
panels combined with a dedicated camera and filter system.

  • 16 MPix Greyscale camera with prime lens for distorti
    on-free imaging
  • LEDs with a range of different wavelengths – camera
    images 16 MPix per channel
  • Filter wheel for up to 6 different filters for filtering
    reflected or emitted light

Wavelength ranges of LEDs in the systemIn a typical setup, the LED array consists of UV, blue, green, yellow, red, deep red, far red and near-infrared wavelength LEDs. Thereby, an advanced analysis of the red edge is enabled as different images in the red range are provided and can be used for ratio calculation. The filter wheel enables filtering for reflectance or fluorescence, e.g. in the chlorophyll fluorescence range.

Multi-channel LED illumination combined with filters enables various imaging settings

  • Channel-wise imaging

  • Combination of channels

  • Chlorophyll autofluorescence

  • Fluorescent biomarkers (e.g. GFP)

  • NDVI and other indices

  • Fluorescence imaging

We bought a LemnaTec LabScanalyzer (the predecessor of PhenoAIxpert) in July 2018. The instrument was delivered to us and installed remotely by a Lemnatec technician that instructed us about the use of the instrument. We were happy about the service: very good communication and excellent instrument set-up. The Lemnatec LabScanalyzer works nicely and is even more user-friendly than we expected. Almost no training of new users is needed. We hope to expand the uses of the instrument to obtain additional phenotyping options in the future, by interacting with LemnaTec.

Prof. Pierdomenico Perata, School of Advanced Studies, Pisa, Italy, Institute of life science

LemnaTec Product Matrix

LemnaTec offers imaging and image processing technologies for several applications that suit for low to high throughput in laboratory, greenhouse or outdoor environments. Technologies are adapted to various sample types and can be combined with automation. All imaging systems are combined with dedicated image processing software, including user-programmable functions and machine learning.

Ask for a quotation or additional information