Scanalyzer Solutions for glasshouses and growth rooms
The LemnaTec Greenhouse Scanalyzer Solutions comprise automated measuring instruments for high-throughput recording of phenotypic data. Imaging and sensing units combined with mechanical automation can be mounted into indoor growth spaces such as glasshouses, growth rooms, climate chambers, or indoor farms.
LemnaTec offers the Greenhouse Scanalyzer in a Plant-to-Sensor configuration, using conveyers that transport the plants to imaging cabinets, or in a Sensor-to-Plant configuration, where a gantry moves the sensors across the plant area.
In the third configuration, we address roots and shoots simultaneously. Licensing the Growscreen Rhizo from Forschungszentrum Jülich GmbH, Germany, we offer an automated root and shoot phenotyping system.
Maize plants in imaging cabinets of Greenhouse Scanalyzer
In the Greenhouse Scanalyzer, each plant is imaged sequentially with multiple camera units, employing different wavelengths in the visible and non-visible spectrum. Besides visible light imaging, LemnaTec offers imaging options in the near-infrared spectrum, hyperspectral, fluorescence, and chlorophyll fluorescence imaging. The result is an unprecedented number of reproducible data points on many aspects of plant health and development.
|Greenhouse Scanalyzer Plant to Sensor - sensor options|
|Visible-light (VIS) Camera Module||Size, count, colour, morphology, texture, movement|
|Near infrared (NIR) Camera Module||Reflectance in the water band at 1450 nm|
|Fluorescence Imaging Module||Fluorescence signals of pigments after appropriate excitation|
|Chlorophyll Fluorescence Kinetics Module||PAM imaging, chlorophyll status and activity|
|Hyperspectral Imaging Module||Spectrally resolved reflectance|
|Multispectral Imaging Module||Reflectance at a series of distinct wavelengths|
|Greenhouse Scanalyzer Plant to Sensor – imaging cabinet dimensions|
|Imaging cabinets are available for different plant sizes|
|Greenhouse Scanalyzer Plant to Sensor – conveyer dimensions|
|Conveyers are available for different sample sizes and weights|
Indoor: X-Y-Z Gantry System
The Indoor X-Y-Z System is a Gantry System made to fit into growth rooms. The gantry is equipped with a sensor change system that allows attaching all cameras and sensors one by one. To do so, cameras will be stored at a station, and the gantry attaches each of them for measuring and places it back afterwards. This sensor-exchange system can overcome space limitations that occur in growth rooms by avoiding the need for a single box containing all sensors at once. Moving the sensors across the area inside the room, the system operates in sensor-to-plant mode and thereby records data of single standing plants, or of canopies, depending on the experimental configuration in the room. Time scheduling for repeated time course measurements with all sensors is available, so that continuous monitoring of the samples with all sensing equipment is achieved.
The following sensors can be used with the indoor gantry system:
- Visible-light (VIS) Camera Module
- Near infrared (NIR) Camera Module
- Infrared (IR) Camera Module
- Chlorophyll Fluorescence Kinetics Module
- Hyperspectral Imaging Module
- Multispectral Imaging Module
- 3D Laser Scanning Module
Combined root- and shoot- phenotyping
While most phenotyping solutions focus on above-ground traits of the plants, roots are usually hidden in soil and thus not accessible to measurements. To make them visible, specific cultivation measures are available. The Growscreen Rhizo uses soil-filled rhizoboxes placed at 45° angle that have a transparent plate where roots are visible for image recording. In collaboration with and under licence of the Forschungszentrum Jülich, LemnaTec offers the Growscreen Rhizo as combined root-and shoot- phenotyping system. Core component is an image acquisition cabinet with high-resolution cameras focused on the roots and the shoots of the plants. Thereby, classical shoot imaging is done simultaneously with precision root imaging. The cabinet is also equipped with dedicated illumination to enable optimal image recording.
A balance and water supply system can be mounted for precise control of soil water content, enabling growth scenarios with different water availability.
Automation technology is available to move in and out the rhizoboxes so that experiments with repeated time-course measurements can be planned. Automation can be done with conveyer-based systems or with magazine automation technology, depending on the local requirements.
Software tools give access to shoot- and root- phenotypic traits such as growth and morphology. Root systems are characterised for root system depth and width and for spatial root distribution, together with root length and width data, rooting angles or total root area. Shoot systems are analysed for height, width, area, and morphological factors, together with colour data.
References from the developers at Forschungszentrum Jülich
Bauke SL, Landl M, Koch M, Hofmann D, Nagel KA, Siebers N, Schnepf A, Amelung W (2017) Macropore effects on phosphorus acquisition by wheat roots – a rhizotron study. Plant and Soil 371:179
Avramova V, Nagel KA, AbdElgawad H, Bustos D, DuPlessis M, Fiorani F, Beemster GTS (2016) Screening for drought tolerance of maize hybrids by multi-scale analysis of root and shoot traits at the seedling stage. Journal of Experimental Botany 67:2453–2466
Gioia T, Nagel KA, Beleggia R, Fragasso M, Ficco DBM, Pieruschka R, Vita P de, Fiorani F, Papa R (2015) Impact of domestication on the phenotypic architecture of durum wheat under contrasting nitrogen fertilization. Journal of Experimental Botany 66:5519–5530
Nagel KA, Putz A, Gilmer F, Heinz K, Fischbach A, Pfeifer J, Faget M, Blossfeld S, Ernst M, Dimaki C, Kastenholz B, Kleinert A-K, Galinski A, Scharr H, Fiorani F, Schurr U (2012) GROWSCREEN-Rhizo is a novel phenotyping robot enabling simultaneous measurements of root and shoot growth for plants grown in soil-filled rhizotrons. Functional Plant Biology 39:891.
Plant carriers on conveyers
To ensure a high degree of homogeneity within treatment groups, LemnaTec’s MOVING FIELD uses conveyers to constantly move plants around in a predefined sequence. This also enables users to optimize plant density and simulate more realistic agricultural growing conditions resulting in more relevant research data.
In phenotypic studies there is an increasing demand for sensor-to-plant platforms, as it is preferable not to move the plants to avoid mechanical stress. For such applications, a motorized gantry can be fitted inside the greenhouse to transport sensors above the plants. The Greenhouse Scanalyzer Sensor-to-Plant can carry a variety of sensors that capture phenotypic properties and sensors for environmental factors.
Greenhouse Scanalyzer Sensor-to-Plant