LabScanalyzer2018-12-06T13:38:51+00:00

LabScanalyzer

Affordable Phenotyping for any laboratory

The LemnaTec Lab Scanalyzer is a versatile instrument for a broad range of phenotyping applications in laboratories. It allows imaging of many different types of biological samples including plants as well as samples in plates:

  • Seedlings and small plants in pots or small trays
  • samples in microtiter plates or petri-dishes, e.g. seeds, leaf disks, insects
  • samples in beakers, e.g. duckweed for ecotoxicology testing
  • detached plant parts, e.g. leaves, fruits, roots, tubers

Lab Scanalyzer standalone and benchtop versions

Lab Scanalyzers consist of cabinets containing high-resolution RGB cameras together with the corresponding illumination. Depending on the configuration, cameras can be mounted in top- and/or side-view position and lights are available as direct- or back-light panels. The unique touch-control software facilitates easy handling of experiments. The software comprises purpose-specific workflows, e.g. seedling growth analysis or duckweed testing, that enable data acquisition and analysis with a few clicks on the user interface. Customised workflows are available on request and can be established interactively. The software generates of standard reports presenting the results to the operator and enables data export in CSV files. Processing of visible light RGB-images delivers data on dimensions, morphology, color, and counts of the biological objects. Dimensions, such as height, width, or projected area, and their change from one measurement to the next give information on growth rates and developmental processes. The table provides an insight of application cases; the actually available features depend on the choice of workflows.

SensorFeatureDescription and application cases
Visible light cameraSizeProjected object area
CountNumber of visually-separated objects
ColorSurface color properties, e.g. greenness or characteristic colorations due to senescence, disease, nutrient deficiency, etc.
MorphologySpatial properties of the object, e.g. compactness, convex hull
TextureSpatial arrangement of color or gray values*
MovementAssignment of object movement or activity using time-lapse imaging

Lab Scanalyzers are available as top-view cabinets or as top-and-side (TS) view cabinets. When working with seedlings and small growing plants, TS cabinets enable monitoring the vertical growth of the samples in addition to the top-down perspective.

  • Top camera
  • Taller plants compared to Bench-Top Lab Scanalyzer
  • On-board analytics
  • 600 mm x 600 mm footprint; 1750 mm height, 100 kg weight

  • Top camera
  • Taller plants compared to Bench-Top Lab Scanalyzer
  • On-board analytics
  • 600 mm x 600 mm footprint; 1750 mm height, 100 kg weight

  • Top and site camera
  • Plant dimensions: max. 300 mm height and 300 mm diameter
  • On-board analytics
  • 2059 mm x 660 mm footprint; 1290 mm height, 190 kg weight

Lab Scanalyzer cabinets can harbor the LemnaTec seed germination workflow and thus serve as Germination Scanalyzer, too. A special version of the Lab Scanalyzer is the Bioluminescence version that comprises a light-tight cabinet to prevent any external light reaching the samples. The images are acquired with a high-sensitivity camera that can record luminescence signals from the sample tissues.

References from customers operating Lab Scanalyzers

Padilla-Chacón D, Peña Valdivia CB, García-Esteva A, Cayetano-Marcial MI, Kohashi Shibata J (2019) Phenotypic variation and biomass partitioning during post-flowering in two common bean cultivars (Phaseolus vulgaris L.) under water restriction. South African Journal of Botany 121:98–104

Białk-Bielińska A, Matzke M, Caban M, Stolte S, Kumirska J, Stepnowski P (2018) Effects of five sulphonamides on duckweed (Lemna minor) after prolonged exposure time and their dependency on photoradiation. The Science of the total environment 618:952–960

Lobiuc A, Vasilache V, Pintilie O, Stoleru T, Burducea M, Oroian M, Zamfirache M-M (2017) Blue and Red LED Illumination Improves Growth and Bioactive Compounds Contents in Acyanic and Cyanic Ocimum basilicum L. Microgreens. Molecules (Basel, Switzerland) 22

Meepagala KM, Briscoe WE, Techen N, Johnson RD, Clausen BM, Duke SO (2017) Isolation of a phytotoxic isocoumarin from Diaporthe eres-infected Hedera helix (English Ivy) and synthesis of its phytotoxic analogs. Pest Manag Sci

Stavropoulou MI, Angelis A, Aligiannis N, Kalpoutzakis E, Mitakou S, Duke SO, Fokialakis N (2017) Phytotoxic triterpene saponins from Bellis longifolia, an endemic plant of Crete. Phytochemistry 144:71–77

The Lab Scanalyzer is available as top- and side-view version, too. This version is particularly dedicated for imaging potted plants up to approximately 400 mm height and 400 mm diameter. One top-down viewing camera and one horizontally viewing camera enable taking images of the samples from all sides.

Lab Scanalyzer top- and side-view version

Samples types
  • Plants in pots or small trays
  • Samples in multiwell plates or petri dishes, e.g. seeds, leaf disks
  • Beakers, e.g. for duckweed
  • Plant parts, e.g. leaves, fruits
Imaging Top view visible light camera OR Top- and side-view visible light cameras

8 Megapixel, 35 mm lens, 29°/21° view angle; other optics on request.

Example for field of view with 35 mm lens:

414 mm x 310 mm at 800 mm distance from lens

258 mm x 194 mm at 500 mm distance from lens

155 mm x 116 mm at 300 mm distance from lens

Illumination Top or bottom OR Top and side
Dimensions Benchtop version: 600 mm x 600 mm footprint; 1128,5 mm height

Standalone version: 600 mm x 600 mm footprint; 1750 mm height

Top- and side-view version: 2059 mm x 660 mm footprint, 1290 mm height

Internal space Benchtop version: approx. 410 mm x 480 mm sample area; 550 mm distance from camera to bottom; max. dimensions of samples depend on optics.

Standalone version: approx. 410 mm x 480 mm sample area; 1100 mm distance from camera to bottom; max. dimensions of samples depend on optics.

Top- and side-view version: approx. 450 mm x 450 mm; 1000 mm distance from top camera and 1200 mm distance from side camera; max. dimensions of samples depend on optics

Weight Benchtop version: 60 kg (approx)

Standalone version: 100 kg (approx)

Top- and side-view version: 190 kg (approx)

Control Integrated PC with touch panel (top view versions) OR dedicated PC for top- and side-view version
Software Process control, image acquisition, data export, data processing

Applications

MORPHOLOGICAL PARAMETERS OF ARABIDOPSIS

Automated quantitative phenotyping of complete plants provides an almost unlimited number of morphological parameters that are easily correlated with biological effects over time. Similar approaches can be adopted for a wide range of other biological applications.

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PARTITIONING TURF IMAGES

Region-based feature extraction focuses on the local distribution of low level features such as colour and texture.

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DUCKWEED DETECTION AND COUNTING OF THALLI

Image workflows are used to monitor the growth of duckweed over time

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