Automated imaging, chlorophyll fluorescence recording, and 3D scanning
The LemnaTec PhenoCenter is the most rapid solution in phenotyping samples in trays. It brings advantages in phenotypic knowledge by combining PAM fluorescence imaging, hyper-/multi-spectral imaging, 3D scanning and imaging in visible and near-infrared range. It is a laboratory-scale, semi-automated system to record and analyze images of plants, leaf disks, fungi, cultures and small organisms such as mosquito larvae or mites.
Cameras move over a sample stage that can contain trays with pots or up to 18 multi-well plates, assessing large numbers of different samples in one run.
The closed cabinet of the PhenoCenter guarantees consistent and reproducible illumination and imaging conditions, suitable for the camera types supplied by LemnaTec. Lighting is adapted to camera requirements and sample properties. Cameras can be easily repositioned to suit different sample geometries. Different optical configurations are available depending on sample types.
Plant trays can be loaded manually into the base module, or they are delivered in an automated tray storage unit that brings them onto the imaging stage subsequently.
The Phenocenter Tray Provider accelerates sample measuring and saves labour. It is an accessory to the Phenocenter Base Module that allows temporally storing of sample trays in a trolley shelf system and automatic transport of all trays into the Base Module for measuring. Transporting in and out the trays, the Tray Provider enables automatically measuring phenotypic parameters of samples in a series of trays.
The trolley shelves are loaded with sample trays by the users and connected to the Tray Provider unit. Thereafter, the Tray Provider works off all presented trays and users can attach further trolley shelves when finished. The trolley shelves have a series of slots for trays and can handle trays of different height, depending on the sample type.
Phenocenter standard configuration for samples up to 20 cm height
|Phenocenter Base Module – camera and sensor options|
|Visible-light (VIS) Camera Module (standard)||Size, count, colour, morphology, texture, movement|
|Near infrared (NIR) Camera Module||Reflectance in the water band at 1450 nm|
|Fluorescence Imaging Module||Fluorescence signals of pigments after appropriate excitation|
|Chlorophyll Fluorescence Kinetics Module||PAM imaging, chlorophyll status and activity|
|Hyperspectral Imaging Module||Spectrally resolved reflectance|
|Multispectral Imaging Module||Reflectance at a series of distinct wavelengths|
|3D Laser Scanning Module||3D point cloud, height and angle|
|Tray Provider||Automatic delivery of sample trays to the Base Module|
|Trolley for tray holders||Carrier for temporally storing and transporting of trays; attachable to the Tray Provider|
|Tray holders with sample adaptors||Shelf plates for the Trolleys that carry the sample trays with sample-specific adaptors|
References from customers operating a predecessor model of the Phenocenter
Campbell ZC, Acosta-Gamboa LM, Nepal N, Lorence A (2018) Engineering plants for tomorrow: how high-throughput phenotyping is contributing to the development of better crops. Phytochem Rev 44:94
Omidbakhshfard MA, Fujikura U, Olas JJ, Xue G-P, Balazadeh S, Mueller-Roeber B (2018) GROWTH-REGULATING FACTOR 9 negatively regulates arabidopsis leaf growth by controlling ORG3 and restricting cell proliferation in leaf primordia. PLoS Genetics 14:e1007484
Brugière N, Zhang W, Xu Q, Scolaro EJ, Lu C, Kahsay RY, Kise R, Trecker L, Williams RW, Hakimi S, Niu X, Lafitte R, Habben JE (2017) Overexpression of RING Domain E3 Ligase ZmXerico1 Confers Drought Tolerance through Regulation of ABA Homeostasis. PLANT PHYSIOLOGY 175:1350–1369
Lucia M. Acosta-Gamboa, Suxing Liu, Erin Langley, Zachary Campbell, Norma Castro-Guerrero, David Mendoza-Cozatl, Argelia Lorence, Acosta-Gamboa LM, Liu S, Langley E, Campbell Z, Castro-Guerrero N, Mendoza-Cozatl D, Lorence A (2017) Moderate to severe water limitation differentially affects the phenome and ionome of Arabidopsis. Functional Plant Biol. 44:94.
Michaud O, Fiorucci A-S, Xenarios I, Fankhauser C (2017) Local auxin production underlies a spatially restricted neighbor-detection response in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America 114:7444–7449
Cameras on camera holders in Phenocenter
The Phenocenter can be equipped with five different camera/sensor types, three of which can be mounted and operated at once. Manual exchange of cameras between experiments is possible. Phenocenters are delivered as standard with visible light cameras, all other camera types are optional accessories.
The visible light camera takes photographs that can be processed for data on sample dimensions and morphology together with colour information.
The laser scanner generates three-dimensional point clouds that can be processed for data on sample dimensions and morphology.
The fluorecence camera takes single level fluorescence images that can be processed for fluorescence intensity. Fluorescence – depending on light and filter – refers to fluorescing substances in the samples, e.g. chlorophyll and its degradation products.
The chlorophyll fluorescence camera, a built-in Imaging PAM from Walz GmbH, takes dynamic chlorophyll fluorescence images that can be processed for photosystem II parameters.
The near-infrared camera takes images of NIR-reflectance that can be processed for NIR intensity. With the 1450 nm narrow bandpass filter, signals relate to water content of the samples.
|Sensors||Visible light imaging: 8.1 Megapixel, f=50 mm
Single level fluorescence: 1.4 Megapixel, f=25 mm
Dynamic chlorophyll fluorescence: Walz Imaging PAM
3D Laser Scanner
NIR imaging: 636 x 508 Pixel, f=35 mm, 1450 nm narrow bandpass filter
Auto-changer can accommodate three sensors out of these five
|Dimensions||Imaging cabinet 1800 x 800 x 1900 mm
Accessories for sample loading on request.
|Power||400 VAC 50Hz|
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