Multispectral and fluorescence imaging
for physiological phenotyping

Multi-view imaging in a series of wavelength combined with options for fluorescence imaging

Phenotyping does not stop at morphological analyses of plants, but it increasingly addresses the physiology. In physiology, signaling processes are key for controlling any steps in development or responses to the environment. When analyzing signaling, biosensor molecules frequently serve to visualize abundancy, localization, or interaction of biochemical compounds. Molecular plant research increasingly uses genetically encoded biosensors. One of the most used biosensors is GFP, of course. While imaging such reporters at cellular scale is well known, there is increasing demand for whole-plant imaging that visualizes presence of biosensors. In an innovative approach, LemnaTec combines multispectral and fluorescence imaging. Using spectral and fluorescence imaging, we obtain physiological information from intact plants. With our advanced spectral imaging tool, we can excite the marker’s fluorescence and record the emission through a filter system. Thereby we can not only visualize the presence and localization of the biosensor, but we can quantify the light emission intensity as measure of the abundance of such sensors. Broader speaking, we can combine classical morphological phenotyping with advanced physiological measurements at whole-plant level. Combined multispectral and fluorescence imaging is available for systems of the PhenoAIxpert family.

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GFP-imaging

The example image shows a GFP-expressing Arabidopsis plant (right) compared to a non-transformed plant (left). The color coding displays recorded fluorescence intensities from blue (low) to red (high). After subtracting the background fluorescence as visible in the control plant, the remaining signal intensity is a measure for GFP abundance in the tissue. The current measurement was done in cooperation with the University of Bonn, INRES, Prof. Dr. Andreas Meyer and Dr. José Ugalde.

Some of the outstanding application possibilities

  • Whole plant imaging in nine wavelengths for multispectral analysis of phenotypes
  • Vegetation indices, e.g. NDVI
  • Analysis of environmental stress and plant diseases
  • Plant nutrition and quality
  • Fluorescence excitation and imaging, e.g. chlorophyll, GFP
  • Biomarker studies

Customers who trust in our solutions

Take the step from morphological to physiological phenotyping!

With our innovative PhenoAIxpert systems combined with the AI-based analytical software a broad range of features describing morphological and physiological phenotypes are in reach.

  • Stress responses
  • Disease resistance
  • Plant quality traits
  • Growth and development
  • Vegetation indices

Marcus Jansen

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Dr. Marcus Jansen
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We will be happy to inform you about further possibilities!

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