Advanced Plant Phenotyping

Phenotypic data for growth and performance studies in research, breeding, and plant treatments

Phenotypes are the outer appearance of organisms and comprise all visible and measurable features that can be assessed from outside without major interfering with the plant. Basically, this comprises counts, dimensions, colors, and other surface properties delivering structural phenotypic information. Moreover, phenotypes include physiological features, such as photosynthetic processes, water status, biochemical properties of the surface, or temperatures.

Phenotypic data are required in research, breeding, agricultural monitoring, in product developments of fertilizers, agrochemicals, or plant adjuvants, and many more applications. For data recording, high-resolution cameras, IR/NIR cameras, fluorescence imaging systems, laser scanners, or hyperspectral imaging systems are applied, and recorded image data are processed with advanced algorithms that extract phenotypic information out of the recordings.

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APPLICATION CASES

SOLUTIONS

  • Phenotyping for environmental stress responses
    Abiotic stresses have large potential of damaging plants, and this is one of the major threats in crop production. Detecting and quantifying stress effects gives a strong advantage in rating plant performance and stress tolerance.
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  • Phenotyping in a mutant study
    A common practice in laboratory tests, e.g. when studying functions of mutations, is growing seedlings on solid media in petri dishes. Such growth assays serve to compare phenotypic properties of mutants and wild types in an easy and fast manner.
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  • Phenotyping in bean breeding
    Beans are a key protein source for large parts of the world population, but bean plant cultivation is threatened by adverse climatic conditions. Breeding aims at improving future bean varieties for better yield performance under environmental stress.
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  • Phenotyping for investigation of water stress response
    Using an HTS Scanalyzer with RGB-, NIR-, and fluorescence cameras, researchers generated 4320 images of Arabidopsis plants in a drought study. Growth responses, water content, and chlorophyll-originating fluorescence signals were used to physiologically characterise the responses of the plants towards water limitation in different severities.
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  • PhenoAIxpert / PhenoAIxpert Pro – the versatile phenotyping system for laboratories
    The versatile phenotyping system for laboratories – your entry to the phenotyping world.
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  • HyperAIxpert – the most flexible multi-sensor laboratory phenotyping system
    The most flexible multi-sensor laboratory phenotyping system for Arabidopsis, seedlings, petri dishes, MTPs, and many more sample types.
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  • PhenoAIxpert HT – the automated versatile phenotyping system (plant-to-sensor)
    Conveyor-based phenotyping solutions for glasshouses, growth rooms, climate chambers, cultivation halls, or indoor farms
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  • CropScore – scalable phenotyping with stationary cameras
    The LemnaTec CropScore phenotyping system is an array of stationary cameras distributed in the growth area. The LemnaTec CropScore phenotyping system is powered by Computomics GmbH, Tübingen, Germany.
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  • Canopy Scanalyzer – the automated versatile phenotyping system (sensor-to-plant) for indoor applications
    Automated high-throughput Canopy Scanalyzers combine rapid automated data recording with advanced analytical software and machine learning.
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  • Field Scanalyzer – the automated versatile phenotyping system (sensor-to-plant) for outdoor applications
    The Field Scanalyzer Gantry System is a 3-axis sensor-to-plant phenotyping system. The design and construction bases on an industrial portal crane system. The x-axis is guided along a rail system underpinned by concrete piles driven into the ground so as to allow natural drainage and no impediments as traditional concrete footings may act as flow barriers.
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Phenotyping – measuring the phenotypes of organisms

SeedAIxpert HT Shelf System

SeedAIxpert HT Shelf System

Phenotyping is central to the human experience of the natural world. Phenotyping drove the domestication events that are hallmarks of the Neolithic revolution, and underlies the yield improvements recognized by modern agriculture. Gregor Mendel phenotyped traits in pea plants to formulate the Laws of Inheritance describing equal segregation, independent assortment, and dominance of alleles.

Increasingly sophisticated phenotyping tools are needed to recognize the trait-improvement potential of genome-wide association studies in areas such as phenotypic differentiation of complex traits, phenotypic plasticity across environments, and phenotypic validation of genomic predictions.

Digital tools are key in state-of-the-art phenotyping. Mendel’s visual scoring was ground-breaking, but in the 21st century computer-assisted sensing and machine learning is the way forward in solution-oriented phenotype measuring.

LemnaTec fulfills this need by developing computer vision tools for high-resolution plant phenotyping in controlled-environments and fields. LemnaTec integrates industrial sensors and illumination with powerful analytical software and robotic automation. Thereby we deliver high-quality digital phenotypic data for agricultural research and product development.

LemnaTec multi-sensor systems measure parameters in 2D images and 3D laser scans across the wavelength spectrum including the visible range, near-infrared, infrared hyperspectral, and PAM fluorescence. The results enable breeders to derive a comprehensive digital phenotype. These data describe plant growth, development, color, geometry, biomass, stress response, disease status, water- and nutrition status, and much more information on structural and physiological traits.

LemnaTec is the market leader in plant phenotyping

Field Scanalyzer

Field Scanalyzer

Plant phenotyping is the quantitative assessment of complex plant traits. It has a broad range of applications in plant research, breeding, product development, and quality assessments.

Since more than 20 years LemnaTec delivers digital solutions that rely on image processing for biological purposes. We address a large range of applications in industry and scientific research.

LemnaTec provides phenotypic data that explain gene functions and that are key to detect QTLs. Our digital phenotyping systems give insight how plants cope with limitation of water and nutrients, how they respond to light or CO2 and how pests and pathogens take influence on plant development. We are phenotyping samples for toxicology, environmental monitoring, and plant management. For breeders and gene banks, seed germination is a central trait for seed viability, which we analyse with digital image processing.

We bought a LemnaTec LabScanalyzer (the predecessor of PhenoAIxpert) in July 2018. The instrument was delivered to us and installed remotely by a Lemnatec technician that instructed us about the use of the instrument. We were happy about the service: very good communication and excellent instrument set-up. The Lemnatec LabScanalyzer works nicely and is even more user-friendly than we expected. Almost no training of new users is needed. We hope to expand the uses of the instrument to obtain additional phenotyping options in the future, by interacting with LemnaTec.

Prof. Pierdomenico Perata, School of Advanced Studies, Pisa, Italy, Institute of life science

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