Bioluminescence imaging

Bioluminescence as reporter for gene activity, plant-microbe-interactions and more

Biomarkers frequently serve to make interactions visible – at molecular level, or at microbial level. Imaging of bioluminescence enables time- and space-resolved monitoring of presence, intensity, and localization of luminescence signals in the sample material.

For this task, LemnaTec has developed PhenoAIxpert ES, a phenotyping system having a high-sensitivity camera that captures weakest light emissions and thus can image luminescence light emitted from the samples. The bioluminescence imaging sensor is combined with high-resolution RGB imaging for standard phenotypic assessments of the analyzed plant material. Images from both sensor systems can be superimposed, thus allowing the analysis of bioluminescence in combination with classical phenotyping.

The PhenoAIxpert ES has a high-sensitivity high-resolution camera with a deep-cooled vacuum CCD sensor. Being one of the most sensitive CCD cameras in the world, the system provides optimized photon collection properties and allows for precise recording of weakest light emissions. The camera is dedicated for long exposure times.

The second camera mounted in the PhenoAIxpert ES is a >12 MPixel RGB industrial grade camera combined with optics and LED illumination optimized for top-view phenotyping of the plant material.

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APPLICATION CASES

SOLUTIONS

  • Imaging of bacteria invading plants
    Since we delivered a PhenoAIxpert ES to the Swammerdam Institute for Life Sciences (SILS) in Amsterdam recently, researchers started their first experiments with the system. With the two cameras mounted inside the PhenoAIxpert ES, they can image both, the growing plants and the bioluminescence emitted from the bacteria that colonize the plants.
    read more …
  • PhenoAIxpert ES
    High sensitivity bioluminescence imaging for plant applications.
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We bought a LemnaTec LabScanalyzer (the predecessor of PhenoAIxpert) in July 2018. The instrument was delivered to us and installed remotely by a Lemnatec technician that instructed us about the use of the instrument. We were happy about the service: very good communication and excellent instrument set-up. The Lemnatec LabScanalyzer works nicely and is even more user-friendly than we expected. Almost no training of new users is needed. We hope to expand the uses of the instrument to obtain additional phenotyping options in the future, by interacting with LemnaTec.

Prof. Pierdomenico Perata, School of Advanced Studies, Pisa, Italy, Institute of life science

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