Bioluminescence Phenotyping

Bioluminescence as reporter for gene activity, plant-microbe-interactions and more

Biomarkers often play a crucial role in visualizing interactions at both the molecular and microbial levels. Bioluminescence imaging provides time- and space-resolved monitoring of luminescence signals, capturing their presence, intensity, and localization within the sample material.

To address this need, LemnaTec has developed the PhenoAIxpert ES, a phenotyping system equipped with a high-sensitivity camera capable of detecting even the weakest light emissions. This enables the imaging of luminescence emitted by the samples. The bioluminescence imaging sensor is paired with a high-resolution RGB camera, which performs standard phenotypic assessments of the plant material. By superimposing images from both sensor systems, researchers can analyze bioluminescence in conjunction with classical phenotyping data.

The PhenoAIxpert ES features a highly sensitive, high-resolution camera with a deep-cooled vacuum CCD sensor, renowned as one of the most sensitive CCD cameras available globally. Its optimized photon collection capabilities allow for precise detection of faint light emissions, even during long exposure times.

In addition, the system includes a second camera: a >12 MP RGB industrial-grade camera, integrated with specialized optics and LED illumination. This camera is optimized for top-view phenotyping, ensuring high-quality imaging of the plant material for comprehensive analysis.

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APPLICATION CASES

SOLUTIONS

  • Imaging of bacteria invading plants
    Since we delivered a PhenoAIxpert ES to the Swammerdam Institute for Life Sciences (SILS) in Amsterdam recently, researchers started their first experiments with the system. With the two cameras mounted inside the PhenoAIxpert ES, they can image both, the growing plants and the bioluminescence emitted from the bacteria that colonize the plants.
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  • PhenoAIxpert ES
    High sensitivity bioluminescence imaging for plant applications.
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